S-layer proteins (SLPs) regulate their extracellular self-assembly by crystallizing when exposed to an environmental trigger. Additionally, we investigated the genetic dependence of localization among divisome proteins and the cell cycle regulation of their transcript and protein levels to gain insight into the control mechanisms underlying their assembly. For questions or comments, contact the SLAC Office of Communications at communications@slac.stanford.edu. Vasant Iyer, SURF Scholar 2015 PhD at University of Pennsylvania At three separate chromosomal sites the CcrM recognition sequence is fully methylated in swarmer cells, becomes hemimethylated upon DNA replication in stalked cells, and does not become remethylated until just prior to cell division. We report here the isolation of the rpoH gene encoding a homolog of the Escherichia coli RNA polymerase sigma32 subunit, the sigma factor responsible for the transcription of heat shock promoters. View details for Web of Science ID 000179629200032. View details for DOI 10.1073/pnas.1220824110, View details for Web of Science ID 000314558100027, View details for PubMedCentralID PMC3562846. Structure of Anabaena flos-aquae gas vesicles revealed by cryo-ET. Heidi Chen in Gill Bejerano & David Kingsley's lab successfully defended her thesis titled Whole-genome comparisons identify enhancers underlying repeated fin evolution in diverse fishes. The P1 promoter fires early in S phase and contains a GAnTC sequence that is recognized by the CcrM DNA methyltransferase. Additionally, as was found for hemE, an upstream untranslated mRNA also extends into the dnaX coding sequence. Bacterial chromosome segregation: Is there a mitotic apparatus? Researchers develop clever algorithm to improve our The bacterial cell has less internal structure and genetic complexity than cells of eukaryotic organisms, yet it is a highly organized system that uses both temporal and spatial cues to drive its cell cycle. Society for Neuroscience 1991-present. Lateral positions of labeled loci at comparable positions along the length of the cell are strongly correlated when the longitudinal locus positions differ by <0.16 m. By combining photoinduced activation of single EYFP fusions and time-lapse imaging, we obtained sub-40 nm resolution images of the filamentous superstructure of the bacterial actin protein MreB in live Caulobacter crescentus cells. The genes that encode the components and regulatory proteins of the Caulobacter crescentus flagellum are transcribed at specific times in the cell cycle. Biomedical Engineering, Washington University in St. Louis Time-lapse microscopy of the location of the chromosomal origin and 10 selected loci in the origin-proximal half of the chromosome showed that during DNA replication, as the replisome sequentially copies each locus, the newly replicated DNA segments are moved in chronological order to their final subcellular destination in the nascent half of the predivisional cell. Our data demonstrate the potential for further development of borinic esters as antibacterial agents as well as leads to explore more specific inhibitors against two essential bacterial enzymes. This positional bias of MCPs within predivisional cells could reflect either a large compartment or membrane domain within the incipient swarmer cell, or a gradient of MCPs, with the highest concentration in the vicinity of the flagellum. View details for Web of Science ID A1996UU11700009. The circuit drives out-of-phase temporal and spatial oscillation of GcrA and CtrA concentrations, producing time- and space-dependent transcriptional regulation of modular functions that implement cell-cycle processes. Using an inactive form of PleC (PleCH610A) that lacks the catalytic histidine residue, we provide evidence that PleC activity is responsible for the asymmetric distribution of CpaE and itself to only one of the two cell poles. B.S. We are a discovery-driven research group working at the interface between developmental biology, bioengineering, and statistical physics. Identification of additional regulatory genes activated by CtrA will serve to directly connect new regulatory modules to the network controlling cell cycle progression. CcrM homologs are widespread in the alpha subdivision of gram-negative bacteria. x@caltech.edu, x=ecriadoh, Willow Coleman Analysis of the requirements for CtrA polar localization and CtrA proteolysis revealed that both processes require a motif within amino acids 1-56 of the CtrA receiver domain, and neither process requires CtrA phosphorylation. View details for Web of Science ID A1990CW01800056. Here, we extend the ability to image subcellular features within bacteria cells to three dimensions based on the introduction of a cylindrical lens in the imaging pathway. The NPI number of this provider is 1114219102 and was assigned on May 2011. Postdoctoral Scholar PodJ is a protein with a single transmembrane domain that exists in two forms, full-length (PodJL) and truncated (PodJS), each appearing during a specific time period of the cell cycle to control different aspects of polar organelle development. The C. crescentus fliL and fliM genes form an operon that is expressed early in the cell cycle. Strain GR131 has wild-type morphology and chromosome number, as assessed by flow cytometry. Using purified protein in different oxidizing/reducing environments, we generate a calibration curve which can be used to analyze in situ measurements. x@caltech.edu, x=csmith3, Stefan Wan The dynamic positioning of signal transduction proteins may contribute to the regulation of polar differentiation at specific times during the bacterial cell cycle. Stephens, C., Reisenauer, A., Wright, R., Shapiro, L. Flagellar assembly in Caulobacter crescentus: A basal body P-ring null mutation affects stability of the L-ring protein, Cell cycle control by an essential bacterial two-component signal transduction protein. Recent advances in cell-imaging technology with subdiffraction resolution have revealed that the bacterial nucleoid is reliably oriented and highly organized within the cell. Positioning different regulatory and signaling proteins at the opposing ends of the predivisional cell produces molecularly distinct daughter cells. Carver, C.M., DeWitt, H.R, Stoja, A.P. Chen, J. C., Hottes, A. K., McAdams, H. H., McGrath, P. T., Viollier, P. H., Shapiro, L. Two independent spiral structures control cell shape in Caulobacter. Dahlberg, P. D., Sartor, A. M., Wang, J., Saurabh, S., Shapiro, L., Moerner, W. E. Integration of cell cycle signals by multi-PAS domain kinases. Ann Zhu, SURF Scholar 2021 BS Chemical Engineering, Caltech 2024 (exp) These technologies take advantage of biomolecules with Cell cycle progression in Caulobacter is governed by a multilayered regulatory network linking chromosome replication with polar morphogenesis and cell division. After graduating he worked for the NASA Marshall Space Flight Center developing non-destructive evaluation techniques for applications related to the US space program. Rice University, Prof. Jerzy Szablowski View details for Web of Science ID A1972M472200040, View details for Web of Science ID A1971J193000005. View details for Web of Science ID 000251336000004, View details for PubMedCentralID PMC2175322.
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